All of the following are TRUE regarding restriction endonucleases EXCEPT
A) they recognize specific palindromic sequences.
B) they may produce staggered ends when they cleave DNA into fragments.
C) they may produce flush ends when they cleave DNA into fragments.
D) they are used in gene cloning to cleave the single stranded regions of the cDNA produced by reverse transcriptase.
E) they are used to cleave plasmid DNA in specific places in gene cloning experiments.

Question

Quizplus · Accepted Answer

Restriction endonucleases are not used to cleave single stranded regions of cDNA produced by reverse transcriptase in gene cloning, rather they are used to cleave double stranded DNA at specific recognition sites.

All of the Following Are TRUE Regarding Restriction Endonucleases EXCEPT