What are the characteristics of proteins that are used for separation by 2-D gel electrophoresis in the first and second dimension respectively?
A) Affinity/charge
B) Mass/affinity
C) Mass/charge
D) PI/mass
E) Shape/charge

Question

Quizplus · Accepted Answer

In the first dimension of 2-D gel electrophoresis, proteins are separated based on their isoelectric point (pI), which is a point where a protein's net charge is zero. In the second dimension, proteins are separated based on their mass.

What Are the Characteristics of Proteins That Are Used for Separation