Hemoglobin A differs from hemoglobin S in having two more anionic groups (both hemoglobins have a molecular weight of approximately 67,000). Which one of the following separation methods is most likely to distinguish between these two
A) Precipitation with ethanol
B) Gel filtration
C) Ultracentrifugation
D) Electrophoresis

Question

Quizplus · Accepted Answer

Electrophoresis separates molecules based on their charge and size differences. As hemoglobin A and hemoglobin S differ in their charge due to the two extra anionic groups in hemoglobin A, electrophoresis is the most likely separation method to distinguish between these two hemoglobins.

Hemoglobin a Differs from Hemoglobin S in Having Two More