You want to engineer a eukaryotic gene into a bacterial colony and have it expressed. What must be included in addition to the coding exons of the gene?
A) the introns
B) eukaryotic polymerases
C) a bacterial promoter sequence
D) eukaryotic ribosomal subunits
E) eukaryotic tRNAs

Question

Quizplus · Accepted Answer

Bacterial colonies use bacterial promoters for transcription, not eukaryotic ones. Therefore, a bacterial promoter sequence must be included in addition to the coding exons of the gene.

Answer: A, B, D, E are incorrect.
 The introns are not necessary for gene expression in bacteria, eukaryotic polymerases are not compatible with bacterial transcriptional systems, and eukaryotic ribosomal subunits and tRNAs are not necessary for protein synthesis in bacteria.

You Want to Engineer a Eukaryotic Gene into a Bacterial