A key advantage of the method using pseudorabies virus relative to that of anterograde and retrograde labeling techniques is that
A) the pseudorabies virus technique can be used in living brains.
B) the pseudorabies virus can identify serial connections among two or more neurons.
C) anterograde and retrograde labeling procedures take weeks as opposed to hours to trace connections.
D) the pseudorabies virus technique provides three-dimensional detail of cell anatomy.
E) levels of the virus in an area are an indication of the relative activity of that area.

Question

Quizplus · Accepted Answer

The pseudorabies virus technique can identify serial connections among two or more neurons, while anterograde and retrograde labeling techniques can only trace connections between two specific target areas. Additionally, pseudorabies virus technique can be used in both living and fixed tissue, and provides detailed information about the distribution of the virus throughout the brain. However, it does not provide three-dimensional detail of cell anatomy, nor does it indicate relative activity of an area based on virus levels.

A Key Advantage of the Method Using Pseudorabies Virus Relative