Which of the following is true of confocal laser scanning microscopy?
A) This technique is limited to a magnification of X100.
B) The confocal laser scanning microscope uses an electron beam to scan a thin slice of tissue.
C) The procedure can be used to visualize living brain cells.
D) This technique can only be used for tissue subjected to formalin.
E) This technique has less resolution than does light microscopy.

Question

Quizplus · Accepted Answer

Confocal laser scanning microscopy can be used to visualize living brain cells, as it allows for imaging of thick specimens with high resolution and without the need for physical sections. Option A is false because the magnification limit depends on the specific instrument and objective lens used. Option B is false because the confocal microscope uses a laser beam, not an electron beam. Option D is false because formalin fixation is not necessary for confocal microscopy. Option E is false because confocal microscopy has better resolution compared to standard light microscopy.

Which of the Following Is True of Confocal Laser Scanning