Which entity or molecule can convert the artificial substrate X-Gal into a blue product?
A) The enzyme encoded by lacZ
B) The enzyme encoded by ori
C) Green florescent protein
D) Ti plasmid from Agrobacterium tumefaciens
E) A restriction enzyme

Question

Quizplus · Accepted Answer

The enzyme encoded by the lacZ gene, called beta-galactosidase, can convert X-Gal into a blue product through a hydrolysis reaction. X-Gal is an artificial substrate that mimics lactose and is commonly used in molecular biology techniques to identify the expression of the lacZ gene. The presence of a blue color indicates the presence of active beta-galactosidase, which is often used as a marker for gene expression or as a reporter for recombinant DNA technology. The other options mentioned are not known to have the ability to convert X-Gal into a blue product.

Which Entity or Molecule Can Convert the Artificial Substrate X-Gal