When using lacZ as a reporter gene, researchers add X-Gal to the substrate.What would happen if they didn't?
A) The transformed cells would die.
B) Untransformed cells would survive.
C)$\beta$-galactosidase would not be produced.
D)$\beta$-galactosidase would be inactivated.
E) One could not easily see which cells had $\beta$-galactosidase.

Question

Quizplus · Accepted Answer

X-Gal is a substrate that, when cleaved by β-galactosidase, produces a blue color. This color allows researchers to visually identify cells that are expressing lacZ, making it easier to screen for positive transformations. Without X-Gal, researchers would not be able to easily see which cells are expressing lacZ. However, the lack of X-Gal would not affect the survival of the cells, the production or inactivation of β-galactosidase, or any other cellular function.

When Using LacZ as a Reporter Gene, Researchers Add X-Gal β\betaβ

When Using LacZ as a Reporter Gene, Researchers Add X-Gal $\beta$