Recombinant DNA technology requires not only getting the DNA into the cell, but also getting it to replicate appropriately.In this process, the specificity of which enzyme can present a challenge?
A) DNA ligase
B) RNA polymerase
C) Replicase
D) Reverse transcriptase
E) DNA polymerase

Question

Quizplus · Accepted Answer

DNA polymerase is responsible for replicating DNA, but it can be specific to certain types of DNA sequences or structures. For example, some DNA polymerases have difficulty replicating through certain regions of DNA that contain secondary structures, such as hairpin loops or G-quadruplexes. This can present a challenge when trying to replicate recombinant DNA containing these regions.

Recombinant DNA Technology Requires Not Only Getting the DNA into the Cell