In the high-throughput sequencing method, DNA is heated to
A) break it into fragments of about 100 nucleotides long.
B) make it single-stranded.
C) make it double-stranded.
D) get synthetic oligonucleotides to anneal to it.
E) chemically modify the bases.

Question

Quizplus · Accepted Answer

In high-throughput sequencing, DNA is denatured or heated to make it single-stranded. This makes it easier for the sequencing machinery to work on the individual strands, and allows for the generation of many more reads at a time.

In the High-Throughput Sequencing Method, DNA Is Heated to