Which of the following techniques involves the manipulation of single-stranded DNA?
A) Restriction digestion
B) Allele-specific oligonucleotide hybridization
C) Gel electrophoresis
D) PCR amplification
E) DNA linkage analysis

Question

Quizplus · Accepted Answer

Allele-specific oligonucleotide hybridization involves the use of short, single-stranded DNA probes that are complementary to specific target sequences in single-stranded DNA. The probes are designed to hybridize only to a specific allele, allowing for detection and analysis of genetic variations. In contrast, restriction digestion, gel electrophoresis, PCR amplification, and DNA linkage analysis all involve the manipulation of double-stranded DNA.

Which of the Following Techniques Involves the Manipulation of Single-Stranded