What method is used to release the O-linked glycan groups from the membrane-associated glycoconjugates shown in 2 in the figure below?
A) enzymatic cleavage using PNGaseF
B) chemical cleavage using a -elimination reaction
C) chemical cleavage using a Schiff base intermediate
D) enzymatic cleavage using -galactosidase

Question

What method is used to release the O-linked glycan groups from the membrane-associated glycoconjugates shown in 2 in the figure below? 
A) enzymatic cleavage using PNGaseF 
B) chemical cleavage using a -elimination reaction 
C) chemical cleavage using a Schiff base intermediate 
D) enzymatic cleavage using -galactosidase

Quizplus · Accepted Answer

The method used to release O-linked glycan groups from membrane-associated glycoconjugates shown in 2 is chemical cleavage using a beta-elimination reaction. This method involves treatment of the glycoprotein with an alkaline reagent, such as sodium hydroxide, which cleaves the glycosidic bond between the glycan and the protein backbone via a beta-elimination reaction. This results in the release of the O-linked glycan group in the form of an alditol (a sugar alcohol).

What Method Is Used to Release the O-Linked Glycan Groups