Which technique can be combined with mass spectrometry to identify protein antigens in large cellular complexes?
A) immunofluorescence
B) Western blot
C) immunoprecipitation
D) ELISA

Question

Quizplus · Accepted Answer

Immunoprecipitation can be combined with mass spectrometry to identify protein antigens in large cellular complexes. This technique involves using an antibody to selectively pull down the target protein complex, followed by mass spectrometry analysis to identify the proteins within the complex. Immunofluorescence and Western blotting can also be used to detect specific proteins, but they do not provide the same level of specificity and sensitivity as immunoprecipitation combined with mass spectrometry. ELISA is a technique used to detect the presence and amount of a specific protein or antigen in a sample, but it does not provide information about the other proteins in a complex.

Which Technique Can Be Combined with Mass Spectrometry to Identify