During a Western blot,the membrane is washed with a blocking solution before being treated with the primary antibody to
A) retain the position of the proteins in the transfer from gel to membrane.
B) facilitate antibody-antigen interactions.
C) decrease nonspecific antibody binding.
D) facilitate a chemical reaction that can be detected.

Question

Quizplus · Accepted Answer

The blocking solution is used to decrease nonspecific antibody binding by covering potential nonspecific binding sites on the membrane, ensuring that the primary antibody binds specifically to the target protein.

During a Western Blot,the Membrane Is Washed with a Blocking