Christian Anfinsen showed in a famous experiment that it is possible to unfold a protein and refold it to obtain a functional protein.Which two reagents were used in this experiment to unfold the protein?
A) detergent and salt
B) urea and -mercaptoethanol
C) acid and base
D) ethanol and -mercaptoethanol

Question

Christian Anfinsen showed in a famous experiment that it is possible to unfold a protein and refold it to obtain a functional protein.Which two reagents were used in this experiment to unfold the protein?
A) detergent and salt 
B) urea and -mercaptoethanol 
C) acid and base 
D) ethanol and -mercaptoethanol

Quizplus · Accepted Answer

Anfinsen used urea and 11ea8d43_ab6d_b417_b445_f15da46cf882_TB6697_11_TB6697_11-mercaptoethanol to unfold the protein in his experiment. Urea helps to disrupt the hydrogen bonds and other non-covalent interactions that stabilize the protein's structure, while 11ea8d43_ab6d_b417_b445_f15da46cf882_TB6697_11_TB6697_11-mercaptoethanol breaks down disulfide bonds. By removing these stabilizing forces, the protein can be unfolded and then refolded into a functional protein.

Christian Anfinsen Showed in a Famous Experiment That It Is