SDS-PAGE separates proteins primarily due to differences in
A)isoelectric point.
B)mass.
C)polarity.
D)solubility.
E)amino acid sequence.

Question

Quizplus · Accepted Answer

SDS-PAGE separates proteins based on their molecular weight. The SDS detergent linearizes the proteins and gives them an overall negative charge, allowing them to be separated by size as they travel through the polyacrylamide gel matrix.

SDS-PAGE Separates Proteins Primarily Due to Differences in