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A Partial Peptide Sequence for the Wild Type and Three  Wild type:met ile arg met asp lys trp... \text { Wild type:\quad met \quad ile \quad arg \quad met \quad asp \quad lys \quad trp... }

Question 32

Essay

A partial peptide sequence for the wild type and three mutant alleles of a gene,PET1,are shown below.Each mutant was caused by a single point mutation.

 Wild type:met ile arg met asp lys trp... \text { Wild type:\quad met \quad ile \quad arg \quad met \quad asp \quad lys \quad trp... }
 Mutant 1:met ile gln asn gly ... \text { Mutant 1:\quad met \quad ile \quad gln \quad asn \quad gly ... }
Mutant 2:met ile arg met gly lys trp...\text {Mutant 2:\quad met \quad ile \quad arg \quad met \quad gly \quad lys \quad trp...}
Mutant 3: met ile ser met asp lys trp...\text {Mutant 3: \quad met \quad ile \quad ser \quad met \quad asp \quad lys \quad trp...}

a)Using the amino acid sequence of the wild type and the three mutants,deduce the exact DNA sequence of the coding strand of the wild-type allele.
b)What type of mutation (transition vs.transversion vs.indel;missense vs.nonsense vs.frameshift)has occurred in each mutant?
c)What consequence will each of the above-mentioned DNA mutations have on protein function?

Correct Answer:

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a)Each mutation helps determine the corr...

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