Hybridization of single-stranded wild-type DNA with DNA from mutations caused by IS elements characteristically shows (through electron microscopy):
A)chi structures.
B)unpaired tails.
C)a single-stranded loop representing IS DNA.
D)a single-stranded loop representing wild-type DNA.
E)theta structures.

Question

Quizplus · Accepted Answer

Hybridization of single-stranded wild-type DNA with DNA from mutations caused by IS elements leads to the formation of a single-stranded loop representing IS DNA, which can be visualized by electron microscopy. This structure is known as a displacement or D-loop. The other options are not observed during this process.

Hybridization of Single-Stranded Wild-Type DNA with DNA from Mutations Caused