A linear DNA molecule has n target sites for restriction enzyme EcoRI.How many fragments will be produced after complete digestion?
A)n - 1
B)n
C)n + 1
D)2n - 1
E)2n + 2

Question

Quizplus · Accepted Answer

Each EcoRI site will produce 2 fragments after digestion. The first cut will produce a fragment of length n, and the second cut will produce n+1 fragments in total. However, we only count the additional fragments produced by each site, not the one where the cut was made. Therefore, there will be n additional fragments produced, giving a total of n+1 fragments.

A Linear DNA Molecule Has N Target Sites for Restriction