In the PCR process,which of the following molecular bonds of DNA are disrupted at high temperatures?
A) ionic bonds
B) hydrogen bonds
C) polar covalent bonds
D) nonpolar covalent bonds
E) glycosidic bonds

Question

Quizplus · Accepted Answer

In PCR, high temperatures (around 95°C) are used to denature the double-stranded DNA template, which disrupts the hydrogen bonds between the complementary base pairs holding the two strands together. This allows the primers to bind to the single-stranded template and initiate DNA synthesis using Taq polymerase, resulting in the amplification of the target DNA sequence.

In the PCR Process,which of the Following Molecular Bonds of DNA