When mutations are available in a gene of interest,the wild-type allele of the gene can be identified by a process called:
A) In vitro hybridization
B) Plaque hybridization
C) Southern hybridization
D) Complementation screening
E) In vitro packaging

Question

Quizplus · Accepted Answer

Complementation screening is the process of identifying the wild-type allele of a gene when mutations are available. This involves introducing a plasmid carrying a copy of the wild-type allele into cells carrying the mutant allele and observing whether the cells exhibit a restored wild-type phenotype. This technique allows for the identification of the functional wild-type allele of the gene of interest. In vitro hybridization, plaque hybridization, Southern hybridization, and in vitro packaging are all techniques used for DNA analysis, but are not specifically designed for identifying the wild-type allele of a gene with available mutations.

When Mutations Are Available in a Gene of Interest,the Wild-Type