The intragenic complementation between the amino terminal fragment of b-galactosidase and the defective DM15 polypeptide which permits the Xgal color test to be utilized without placing the entire lacZ gene in the pUC vectors is called:
A) Forced cloning
B) a-complementation
C) Blue/white color selection
D) In situ hybridization
E) Complementation screening

Question

Quizplus · Accepted Answer

The intragenic complementation between the amino terminal fragment of b-galactosidase and the defective DM15 polypeptide is known as a-complementation. This allows for the Xgal color test to be utilized without the full lacZ gene by introducing only the amino terminal fragment into the pUC vectors. Forced cloning and in situ hybridization are not related to this process, and complementation screening is a broader term that refers to any method used to identify functional complementation between two mutant genes. The blue/white color selection is related to the Xgal color test, but is not a term used to describe the specific interaction between the amino terminal fragment of b-galactosidase and the defective DM15 polypeptide.

The Intragenic Complementation Between the Amino Terminal Fragment of B-Galactosidase