Find k_cat for a reaction in which V_max is 4 × 10^-4 mol•min^-1 and the reaction mixture contains one microgram of enzyme (the molecular weight of the enzyme is 200,000D). A)2 × 10^-11 min^-1 B)8 × 10⁷ min^-1 C)8 × 10⁹ min^-1 D)2 × 10^-14 min^-1

Question

Quizplus · Accepted Answer

To find k_cat, use the formula k_cat = V_max / [E]. First, convert 1 microgram of enzyme to moles: (1 × 10^-6 g) / (200,000 g/mol) = 5 × 10^-12 mol. Then, k_cat = (4 × 10^-4 mol/min) / (5 × 10^-12 mol) = 8 × 10⁷ min^-1.

Find Kcat for a Reaction in Which Vmax Is 4

Find K_cat for a Reaction in Which V_max Is 4