In step 2 (above)in the identification of the colony that contains a targeted gene,____.
A) bacterial cells are cloned
B) bacterial colonies are transferred
C) bacterial cells are ruptured
D) DNA is lysed
E) cells with the targeted gene are identified

Question

In step 2 (above)in the identification of the colony that contains a targeted gene,____.  
A) bacterial cells are cloned
B) bacterial colonies are transferred
C) bacterial cells are ruptured
D) DNA is lysed
E) cells with the targeted gene are identified

Quizplus · Accepted Answer

In step 2, bacterial colonies containing the targeted gene are transferred from the original plate onto a new plate containing agar media. This is done to isolate a pure colony for further analysis. Therefore, the correct choice is B. Option A is incorrect because bacterial cells are cloned in previous steps. Option C is incorrect because bacterial cells are not ruptured in this step. Option D is incorrect because DNA lysis is not required in this step. Option E is incorrect because cells with the targeted gene are not identified in this step.

In Step 2 (Above)in the Identification of the Colony That