In the Michaelis-Menten mechanism of enzyme reaction, the Michaelis constant,K_M,Is A) K_M = k₁/k₂. B) K_M = k₁'/k₂. C) K_M = (k₁' + k₂)/k₁. D) K_M = k₁' + k₂. E) K_M = k₁/k₁'.

Question

Quizplus · Accepted Answer

According to the Michaelis-Menten equation, v = (Vmax [S]) / (Km + [S]), where Km is the Michaelis constant. Km is equal to the rate constant of the enzyme-substrate complex formation (k1) plus the rate constant of the enzyme-substrate complex breakdown (k-1), divided by the rate constant of the enzyme-substrate complex breakdown (k-1). Therefore, Km = (k1 + k-1) / k-1, which simplifies to Km = k1/k-1 + 1. Substituting k2 for k-1, the equation becomes Km = (k1 + k2) / k2. Choice C is the only option that matches this equation.

In the Michaelis-Menten Mechanism of Enzyme Reaction, the Michaelis Constant,KM,Is

In the Michaelis-Menten Mechanism of Enzyme Reaction, the Michaelis Constant,K_M,Is