The steps involved in a single PCR cycle,in the correct order,are
A) cool to allow DNA strands to anneal, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
B) heat to denature DNA strands, cool to allow primer to anneal and to allow DNA polymerase to copy DNA.
C) heat to denature DNA strands and for primer to anneal, cool to allow DNA polymerase to copy DNA.
D) cool to denature DNA strands, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
E) cool to allow primer to anneal, heat to denature DNA strands, cool to allow DNA polymerase to copy DNA.

Question

Quizplus · Accepted Answer

The PCR cycle starts with heating the DNA to denature or separate the double-stranded DNA into single-stranded DNA. Then, the reaction is cooled to allow the primers to anneal, or bind, to the complementary sequence on each of the single-stranded DNA templates. Finally, the reaction is heated again to activate the DNA polymerase enzyme, which copies the DNA template to form a new double-stranded DNA molecule. Therefore, the correct order of steps is heat to denature DNA, cool to allow primer annealing and DNA polymerase extension, and then heat to activate the DNA polymerase. Choice B is the only one that uses this correct order of steps.

The Steps Involved in a Single PCR Cycle,in the Correct