In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?
A)Sterile swab, loop dilution, TSB
B)Hockey stick, spread plate technique, and 4 degree Celsius incubation temperature
C)Loop dilution, TSA, hockey stick, 37 degree Celsius incubator
D)Inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator
E)Sterile swab, streak plate method, 37 degree Celsius incubator

Question

Quizplus · Accepted Answer

The best choice to isolate single colonies would be the inoculating loop, incinerator, streak plate method, and the 37 degree Celsius incubator. The inoculating loop is used to transfer a small amount of the mixed culture onto the agar surface of the streak plate. The incinerator is used to sterilize the loop between transfers to prevent contamination. The streak plate method is a technique that dilutes the bacteria on the plate and allows for isolated colonies to form. Finally, the 37 degree Celsius incubator provides a suitable temperature for bacterial growth.

In Lab, Tom Was Given a Mixed Culture