How will E. coli appear if the mordant is not applied?
A) Gram-positive
B) Gram-negative
C) Gram-variable
D) Colorless
E) None of these

Question

Quizplus · Accepted Answer

Without the mordant, the crystal violet stain will not be fixed to the peptidoglycan layer of the Gram-negative cell wall, and the safranin counterstain will dye the cell pink or red instead of being masked by the crystal violet. This results in a Gram-negative appearance.

How Will E