At the end of the Gram stain, gram-positive bacteria will be seen as purple cells.

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Gram-positive bacteria have a thick peptidoglycan layer in their cell walls, which retains the crystal violet stain during the Gram staining procedure, resulting in purple cells under the microscope. Gram-negative bacteria, on the other hand, have a thinner peptidoglycan layer and an outer membrane that can be disrupted by the decolorizing step, causing them to appear pink or red after the counterstaining with safranin.

At the End of the Gram Stain, Gram-Positive Bacteria Will