What is the correct sequence for a Gram stain?
A)Gram's iodine, Crystal Violet, Alcohol, Safranin
B)Crystal Violet, Safranin, Alcohol, Gram's iodine
C)Crystal Violet, Gram's iodine, Alcohol, Safranin
D)Safranin, Crystal Violet, Gram's iodine, Alcohol
E)Alcohol, Crystal Violet, Safranin, Gram's iodine

Question

Quizplus · Accepted Answer

The correct sequence for a Gram stain is Crystal Violet, Gram's iodine, Alcohol, Safranin. This sequence allows for the differentiation between gram-positive bacteria (which retain the crystal violet stain) and gram-negative bacteria (which are decolorized by the alcohol). Gram's iodine acts as a mordant, intensifying the stain. Finally, safranin is used as a counterstain to stain any remaining gram-negative bacteria. Therefore, choice C is the correct answer as it follows the correct sequence for the Gram stain.

What Is the Correct Sequence for a Gram Stain