Which of the following sequences of events in the production of recombinant DNA are in the correct order?
A) mix DNA with plasmid DNA, cut with restriction enzymes, add DNA ligase
B) add DNA ligase, cut DNA with restriction enzymes, mix DNA with plasmid DNA
C) cut DNA with restriction enzymes, mix DNA with plasmid DNA, add DNA ligase
D) mix DNA with plasmid DNA, add DNA ligase, cut with restriction enzymes
E) cut with restriction enzymes, add DNA ligase, mix DNA with plasmid DNA

Question

Quizplus · Accepted Answer

In order to make recombinant DNA, it is necessary to cut the DNA with a restriction enzyme before mixing it with plasmid DNA, which has been cut with the same restriction enzyme. Then, DNA ligase is added to link the DNA strands.

Which of the Following Sequences of Events in the Production