If you have a double stranded synthetic piece of DNA that you want to attach to a PCR product,what enzyme would you use?
A)Taq DNA polymerase
B)reverse transcriptase
C)DNA ligase
D)DNA polymerase
E)restriction enzyme

Question

Quizplus · Accepted Answer

The enzyme DNA ligase is the best choice for attaching the synthetic piece of DNA to the PCR product. DNA ligase catalyzes the formation of phosphodiester bonds between adjacent nucleotides in DNA strands, which allows for the covalent attachment of the synthetic piece to the PCR product. The other enzymes listed do not have the ability to directly attach two separate DNA molecules together.

If You Have a Double Stranded Synthetic Piece of DNA