Southern blot is a method for ____.
A) amplifying DNA segments using cycles of denaturation, annealing to primers, and DNA polymerase-directed DNA synthesis
B) transferring DNA fragments from a gel to a membrane filter and used in hybridization experiments
C) determining the nucleotide sequence of a DNA molecule
D) producing several clones from a single cell by placing the cell in a Petri dish and allowing it to go through several divisions
E) detecting mutant genes or differences in the pattern of gene expression

Question

Quizplus · Accepted Answer

Southern blot is a technique used to transfer DNA fragments from a gel to a membrane filter, and then used in hybridization experiments to detect mutant genes or differences in the pattern of gene expression. It is named after Edward M. Southern, who developed the method in 1975.

Southern Blot Is a Method for ____