The order of reagents in the Gram stain reaction are
A)safranin,alcohol,methylene blue,iodine.
B)crystal violet,iodine,alcohol,safranin.
C)methylene blue,alcohol,safranin.
D)crystal violet,alcohol,iodine,safranin.

Question

Quizplus · Accepted Answer

The correct order of reagents in the Gram stain reaction is crystal violet, iodine, alcohol, and safranin. This is because crystal violet is used to stain both Gram-positive and Gram-negative bacteria, iodine is added to fix the crystal violet to the bacterial cell wall, alcohol is used to wash away the crystal violet from Gram-negative bacteria as they have a thinner peptidoglycan layer in their cell wall, and safranin is used to counterstain Gram-negative bacteria so that they can be easily differentiated from Gram-positive bacteria.

The Order of Reagents in the Gram Stain Reaction Are