Replica plating
A)is useful for direct selection.
B)is useful for identifying auxotrophs.
C)uses media on which the mutant will not grow and the parental cell typewill.
D)is used to store strains of bacteria.
E)is useful for identifying auxotrophs AND uses media on which the mutant will not grow and the parental cell typewill.

Question

Quizplus · Accepted Answer

Replica plating is a technique used to identify auxotrophic mutants by transferring colonies from a master plate (containing all the strains) to selective media plates. The plates contain media on which the mutant will not grow and the parental cell type will, allowing for the identification of mutants that cannot grow on the selective media. Therefore, E is the best choice as it identifies auxotrophs and uses media on which the mutant will not grow and the parental cell type will.

Replica Plating