The size of the amplified DNA fragment generated during PCR is determined by
A)how many cycles are performed.
B)the size of the template DNA.
C)the location to which the primers anneal.
D)how much Taq is used.

Question

Quizplus · Accepted Answer

The size of the amplified DNA fragment is determined by the location to which the primers anneal. The primers define the start and end points of the PCR amplification, and the length of the product is determined by the distance between them on the template DNA. The other options, such as the number of cycles, the size of the template DNA, and the amount of Taq polymerase used, can affect the amount and yield of the PCR product, but not the size. Therefore, choice C is the best answer.

The Size of the Amplified DNA Fragment Generated During PCR