If the lacZ gene is functional it produces an enzyme known as _____________,which allows the bacterial cell to metabolize the sugar,X-gal.
A)beta-glucosidase
B)beta-xylosidase
C)gamma-endonuclease
D)alpha-galactosidase
E)beta-galactosidase

Question

Quizplus · Accepted Answer

The lacZ gene produces an enzyme known as beta-galactosidase, which breaks down the disaccharide lactose into glucose and galactose. Additionally, beta-galactosidase can also break down X-gal, a common substrate used in blue/white screening assays. Therefore, if the lacZ gene is functional and producing beta-galactosidase, the bacterial cell can metabolize X-gal and produce a blue color.

If the LacZ Gene Is Functional It Produces an Enzyme