In Sanger sequencing, what causes DNA synthesis to terminate at a specific base?
A)chemicals that cleave DNA after particular bases
B)fluorescent chemicals
C)nucleotide triphosphates that lack a base
D)nucleotide triphosphates that lack a hydroxyl at the 3' position
E)nucleosides that lack a 5' phosphate

Question

Quizplus · Accepted Answer

In Sanger sequencing, dideoxy nucleotides (ddNTPs) are used along with normal nucleotides (dNTPs) during DNA synthesis. Dideoxy nucleotides lack a hydroxyl group at the 3' position, which is required for further nucleotide addition during DNA synthesis. This results in termination of DNA synthesis at the specific base where the ddNTP is incorporated. The incorporation of ddNTPs occurs at a low ratio compared to dNTPs, resulting in a series of synthesized DNA fragments with different lengths ending specifically at each base.

In Sanger Sequencing, What Causes DNA Synthesis to Terminate at a Specific