How could PCR be used for the detection of a SNP?
A)PCR coupled with Southern blotting using one of the primers as a probe.
B)The PCR reaction would need to be sequenced.
C)The PCR reaction could be subjected to agarose gel electrophoresis to determine the presence and size of the fragment.
D)If a fragment is amplified then the SNP is present, no other techniques would have to be used

Question

Quizplus · Accepted Answer

PCR alone cannot determine the presence of a SNP. The PCR product would need to be sequenced to identify if a SNP is present within the amplified region. Southern blotting and agarose gel electrophoresis may only provide information about the size and presence of the PCR product, but not specifically if a SNP is present.

How Could PCR Be Used for the Detection of a SNP