Which of the Following Is a Problem for the Use

Which of the following is a problem for the use of a microscope in the examination of brain tissue?

A) A light microscope cannot resolve fine synaptic details such as synaptic vesicles.
B) A light microscope can magnify an image up to X1500, which is sufficient to view some details such as synaptic vesicles.
C) Beams of electrons passed through a thin brain section cast a dark shadow on photographic film.
D) A light microscope shows better resolution than does an electron microscope.
E) A light microscope shows three-dimensional details, which makes for a more difficult histological analysis.