Fluorescence microscopy of intact organisms or large cells results in the generation of blurred images.Which of the following is NOT a technique that reduces the out-of-focus signal that causes blurring?
A)two-photon excitation microscopy
B)confocal microscopy
C)fluorescence recovery after photobleaching (FRAP)
D)deconvolution microscopy

Question

Quizplus · Accepted Answer

Fluorescence recovery after photobleaching (FRAP) is a technique used to study the dynamic behavior of fluorescently labeled molecules in living cells, but it does not reduce the out-of-focus signal that causes blurring. A, B, and D are techniques that reduce out-of-focus signal to improve image resolution.

Fluorescence Microscopy of Intact Organisms or Large Cells Results in the Generation