To visualize cells by immunofluorescence microscopy,the cells must be:
A)placed in a vacuum.
B)living.
C)sectioned.
D)permeabilized.

Question

Quizplus · Accepted Answer

Permeabilization is necessary to allow antibodies or other probes to enter into the cells and bind to specific targets, which can then be visualized under the fluorescence microscope. Living cells also need to be fixed before staining to prevent loss or distortion of cellular structures, but sectioning or vacuum is not required for immunofluorescence microscopy.

To Visualize Cells by Immunofluorescence Microscopy,the Cells Must Be