When studying a new protein,it is useful to generate an antibody against it to tag it in experiments designed to study its function and localization.Which of the following is NOT a step needed to generate this new antibody?
A)Mouse spleen cells are fused with myeloma cells.
B)Cells are selected for their ability to divide and grow in the absence of purines in the medium.
C)Mice are injected with the antibody that will tag the new protein.
D)The spleen is isolated from a laboratory mouse.

Question

Quizplus · Accepted Answer

Injecting mice with the antibody that will tag the new protein is not a step needed to generate a new antibody. The steps that are needed include isolating the spleen from a laboratory mouse, fusing mouse spleen cells with myeloma cells, selecting cells for their ability to divide and grow in the absence of purines in the medium, and then testing the resulting antibodies for their specificity and affinity towards the new protein.

When Studying a New Protein,it Is Useful to Generate an Antibody