Methods for breaking proteins into smaller peptides include all of the following except:
A) Digestion with chymotrypsin
B) Cyanogen Bromide treatment
C) Digestion with Trypsin
D) Edmann degradation
E) All of the above create short peptides suitable for sequencing

Question

Quizplus · Accepted Answer

Edman degradation is a method for sequencing amino acids in a peptide, not for breaking proteins into smaller peptides. It sequentially removes one residue at a time from the amino end of a peptide, allowing for the identification of the amino acid sequence, rather than cleaving the protein into smaller peptides.

Methods for Breaking Proteins into Smaller Peptides Include All of the Following