You make two different kinds of metagenomes-one to measure diversity from only 16S rRNA DNA sequences, and one from the entire DNA that does not require amplification. You observe 1,000 times more 16S rRNA gene sequences from the first metagenome than the second. However, you find that the number of species according to the 16S rRNA gene sequences is 10 times more in the second metagenome than the first. Why?
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