Which molecule is used for hybridization to DNA bound on a DNA microchip?
A) DNA
B) RNA
C) mRNA
D) cDNA
E) any of the above

Question

Quizplus · Accepted Answer

cDNA (complementary DNA) is used for hybridization to DNA bound on a DNA microchip. cDNA is synthesized from mRNA using reverse transcriptase, and it represents the complement of the mRNA sequence. The cDNA can then be labeled and hybridized to DNA microarrays to identify genes that are expressed in a particular sample. DNA, RNA, and mRNA are not used for hybridization to DNA microchips.

Which Molecule Is Used for Hybridization to DNA Bound on a DNA