Select all advantages of RNA-Seq as compared to DNA microarrays.
A) Better detection of transcripts present at low levels
B) Identifies boundaries of introns and exons to determine splice variants
C) More accurately quantifies the amount of each transcript
D) Does not require isolation of RNA from the cell
E) Does not require cDNA synthesis

Question

Quizplus · Accepted Answer

A) RNA-Seq has a higher dynamic range and sensitivity than DNA microarrays, allowing for better detection of low-abundance transcripts. 
B) RNA-Seq can distinguish between different transcript isoforms by identifying the boundaries of introns and exons. 
C) RNA-Seq can provide more accurate quantification of transcript abundance than DNA microarrays.
D) Isolation of RNA is still necessary for RNA-Seq.
E) cDNA synthesis is necessary for RNA-Seq, but not for DNA microarrays.

Select All Advantages of RNA-Seq as Compared to DNA Microarrays