In Pavlov's experiment,the conditioned stimulus was the tuning fork tone.

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In Pavlov's classic experiment, he conditioned dogs to salivate at the sound of a tuning fork by repeatedly pairing the sound with the presentation of food, until the dogs began to associate the sound of the fork with the expectation of food. As a result, the tuning fork tone became a conditioned stimulus that could elicit a conditioned response (salivation) without the presence of food.

In Pavlov's Experiment,the Conditioned Stimulus Was the Tuning Fork Tone