You are observing the rate of an enzymatic reaction.You continue to add substrate in higher and higher concentrations,but eventually you see the rate of reaction plateau,no further increases in the rate of the reaction could be observed.Which of the following are plausible explanations for your observation (select all that apply)?
A)The enzyme has become saturated.
B)The substrate solution has become contaminated with an allosteric inhibitor.
C)The enzyme molecules are being used up and broken down by the reaction.
D)Over time,the substrate molecules are denaturing.
E)The temperature of the room needs to increase to increase the rate of reaction.

Question

Quizplus · Accepted Answer

A) When an enzyme becomes saturated, it means that all of its active sites are occupied by substrate molecules. At this point, adding more substrate won't increase the rate of reaction because there are no free active sites left for additional substrate molecules to bind to.B) An allosteric inhibitor binds to an enzyme at a site other than the active site and changes the enzyme's shape, making it less active or inactive. This could explain why adding more substrate doesn't increase the reaction rate, as the enzyme's activity is being reduced by the inhibitor.

You Are Observing the Rate of an Enzymatic Reaction