Place the basic steps involved in a molecular hybridization assay in sequence from first to last in the order in which they would be performed.
A)Heating sample to obtain single-stranded target nucleic acid,hybridization of target to probe,detection of hybridization,labeling of probe
B)Labeling of probe,heating sample to obtain single-stranded target nucleic acid,hybridization of target to probe,detection of hybridization
C)Hybridization of target to probe,detection of hybridization,labeling of probe,heating sample to obtain single-stranded target nucleic acid
D)Hybridization of target to probe,labeling of probe,heating sample to obtain single-stranded target nucleic acid,detection of hybridization

Question

Quizplus · Accepted Answer

The basic steps in a hybridization assay include:
1.Production and labeling of single-strand nucleic acid probe
2.Preparation of single-strand target nucleic acid
3.Mixture and hybridization of target and probe nucleic acid
4.Detection of hybridization

Place the Basic Steps Involved in a Molecular Hybridization Assay